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B016095 - LABORATORY OF CHEMISTRY FOR BIOLOGY
Main information
Teaching Language
Course Content
Suggested readings
Learning Objectives
Prerequisites
Teaching Methods
Further information
Type of Assessment
Course program
Sustainable Development Goals 2030
Academic Year 2023-24
Course year
Second year - Second Semester
Belonging Department
Biology (BIO)
Course Type
Single education field course
Scientific Area
CHIM/03 - GENERAL AND INORGANIC CHEMISTRY
Credits
3
Teaching Hours
36
Teaching Term
19/02/2024 ⇒ 14/06/2024
Attendance required
No
Type of Evaluation
Final Grade
Course Content
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Course program
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Lectureship
- Last names A-L CALDERONE VITO
- Last names M-Z MASSAI LARA
Teaching Language - Last names A-L
Italian
Course Content - Last names A-L
Introduction to chemistry laboratory. Handling of chemicals and laboratory techniques. Safety rules. Redox reactions. Chemical potential. Physical states of matter. Separation of molecular species from a mixture. Acid-base equilibria. Buffer solutions. Acid-base titrations. Biomolecules, simple and complex sugars and proteins. Tests of Fehling and Lugol and Biuret reaction. Chromatography and its applications in biochemistry.
Suggested readings - Last names A-L (Search our library's catalogue)
'Chimica’ I. Bertini, C. Luchinat, F. Mani, Casa editrice Ambrosiana; ‘Stechiometria’ I. Bertini, F. Mani, Casa editrice Ambrosiana; ‘Biochimica’ A. L. Lehninger, Zanichelli.
Learning Objectives - Last names A-L
Acquire basic knowledge and methodologies required in a chemistry laboratory, with particular attention to biochemical aspects.
Prerequisites - Last names A-L
Courses required: General and inorganic Chemistry
Teaching Methods - Last names A-L
Total number of hours for Lectures (hours): 8
Total number of hours for Laboratory practice : 24
Further information - Last names A-L
Attending laboratory practical lessons is mandatory
Type of Assessment - Last names A-L
Written exam
Course program - Last names A-L
Introduction to chemistry laboratory. Handling of chemicals and laboratory techniques. Safety rules. Redox reactions. Chemical potential. Physical states of matter. Separation of molecular species from a mixture. Acid-base equilibria. pH. Buffer solutions. Chromatography and its applications in biochemistry. Biomolecules, simple and complex sugars and proteins.
Copper(II) reduction by iron. Separation of benzoic acid from a mixture. pH measurement. Titration of a oxalic acid solution with sodium hydroxide. TLC chromatographic separation of vegetal pigments. Recognition assays of simple reducing sugars (Fehling), complex sugars (Lugol) and proteins (Biuret reaction).