ITA | ENG
B016090 - MICROBIOLOGY AND LABORATORY
Main information
Teaching Language
Course Content
Suggested readings
Learning Objectives
Prerequisites
Teaching Methods
Type of Assessment
Course program
Academic Year 2020-21
Course year
Second year - First Semester
Belonging Department
Biology (BIO)
Course Type
Single education field course
Scientific Area
BIO/19 -
Credits
9
Teaching Hours
76
Teaching Term
14/09/2020 ⇒ 23/12/2020
Attendance required
No
Type of Evaluation
Final Grade
Course Content
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Course program
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Lectureship
Mutuality
Course teached as:
B016090 - MICROBIOLOGIA CON LABORATORIO
3-years First Cycle Degree (DM 270/04) in BIOLOGICAL SCIENCES
B016090 - MICROBIOLOGIA CON LABORATORIO
3-years First Cycle Degree (DM 270/04) in BIOLOGICAL SCIENCES
Teaching Language
Italian
Course Content
The aim of the course is to offer a basic knowledge of the microbial world and the techniques used for its study.
Suggested readings (Search our library's catalogue)
Madigan, M.T., K.S. Bender, D.H. Bucley, D.A. Stahl. 2016 – Brock Biologia dei microrganismi: Microbiologia generale, ambientale e industriale – Pearson Italia, Milano-Torino.
M. Willet, M. Sherwood, J. Woolverton. 2009 – Prescott Microbiologia – McGraw-Hill, Milano
M. Willet, M. Sherwood, J. Woolverton. 2009 – Prescott Microbiologia – McGraw-Hill, Milano
Learning Objectives
Microbial terminology and molecular aspects of the prokaryotic cell; pure cultures, bacterial growth and growth factors; genetics and microbial metabolism. Growth, isolation, enrichment and characterization of microorganisms; measurement of bacterial cell concentration; sterilization, antibiotic assays, use of instrumentation for microbiological studies. Understanding of the importance of microorganisms in nature and their use.
Prerequisites
Required courses: none
Recommended courses: Genetics, Biochemistry
Recommended courses: Genetics, Biochemistry
Teaching Methods
Lectures and laboratory
Type of Assessment
Oral or written examination. It will be evaluated the knowledge of course topics and the ability to make connections with what learned in the first two years.
Course program
The bacterial cell. Capsule; flagella; cell wall: structure and biosynthesis; membrane; genome. Methods of bacterial identification and classification.
Fungi. Yeast: growth cycle and industrial applications.
Sterilization. Growth media. Pure cultures. Gram staining. Measurement of cell mass and cell number. Bacterial growth curve. Growth factors.
Carbon and energy sources. Fermentation, aerobic and anaerobic respiration. Photosynthetic metabolism. Carbon, nitrogen and sulphur biological cycles. Regulation of bacterial metabolism. Bacterial endospore.
Microbial genetics: transformation, conjugation and transduction. Plasmids. Transposons. Restriction and modification.
Antibiotics producers. Antibiotics mechanism of action. Sulphonamide. Penicillins and other antibiotics acting on cell wall. Antibiotics inhibitors of nucleic acids and proteins. Antifungi. Antiviral molecules. Antibiotic resistance.
Structure and classification of viruses. Viral infection. Lytic and lysogenic phages. Animal viruses.
Virulence factors of pathogenic microorganisms. Microbial toxins. Koch postulates. Host defences. Vaccines.
Examples of industrial microbiology
Fungi. Yeast: growth cycle and industrial applications.
Sterilization. Growth media. Pure cultures. Gram staining. Measurement of cell mass and cell number. Bacterial growth curve. Growth factors.
Carbon and energy sources. Fermentation, aerobic and anaerobic respiration. Photosynthetic metabolism. Carbon, nitrogen and sulphur biological cycles. Regulation of bacterial metabolism. Bacterial endospore.
Microbial genetics: transformation, conjugation and transduction. Plasmids. Transposons. Restriction and modification.
Antibiotics producers. Antibiotics mechanism of action. Sulphonamide. Penicillins and other antibiotics acting on cell wall. Antibiotics inhibitors of nucleic acids and proteins. Antifungi. Antiviral molecules. Antibiotic resistance.
Structure and classification of viruses. Viral infection. Lytic and lysogenic phages. Animal viruses.
Virulence factors of pathogenic microorganisms. Microbial toxins. Koch postulates. Host defences. Vaccines.
Examples of industrial microbiology