Cell cultures. Mathematical models of growth for cell populations. Cell proliferation study. Molecular regulation of cell cycle and differentiation. Molecular mechanisms of translational control. Embrional and adult stem cells: their use in regenerative biomedicine.
Primary cultures and in vitro maintenance of cell lines.
Methods for evaluating cell proliferation.
Autoradiography.
Methods to obtain single cell suspensions.
Immunohistochemical evaluation of proliferation parameters.
Biology and terminology of cell cultures.
Molecular basis of cell cycle.
Flow cytometry techniques.
Molecular mechanisms of translational control.
Embrional and adult stem cells: their use in regenerative biomedicine.
Prerequisites
Courses required: none
Courses recommended: Cytology and Histology, Biochemistry
Teaching Methods
Lectures
Laboratory
Type of Assessment
Oral examination
Course program
Cells and tissues. Primary cultures. Culture manipulation methods. In vitro and in vivo analysis of cell proliferation. Cell kinetics: duration of the phases of cell cycle, potential and actual doubling time, growth fraction cell loss. Mathematical model for growth of cell populations. Molecular basis of cell cycle regulation. Cell differentiation. Stem cells: sources, characterization and use for therapeutic purpose. Molecular mechanisms of translational control.