Cell cultures. Cell kinetics: duration of the phases of cell cycle, potential and actual doubling time, growth fraction, cell loss. Mathematical models of growth for healthy and neoplastic cell populations. Molecular regulation of cell cycle and differentiation.
Embrional and adult stem cells: their use in regenerative biomedicine.
Proliferative activity, turn over and functional differentiation in models of tissues: epidermis, intestine, bone marrow. Effects of chemical, physical and biologic
Biology and terminology of cell cultures.
Molecular basis of cell cycle.
Cell kinetics parameters in healthy and neoplastic tissues.
Flow cytometry techniques.
Cell kinetics in models of healthy tissues.
Use of biological parameters for the study of human tumour aggressiveness.
Primary cultures and in vitro maintenance of cell lines.
Methods for evaluating cell proliferation.
Autoradiography.
Methods to obtain single cell suspensions.
Immunohistochemical evaluation of proliferation parameters.
Prerequisites
Courses required: none
Courses recommended: Cytology and Histology, Biochemistry
Teaching Methods
Lectures
Laboratory
Type of Assessment
Oral examination
Course program
Cells and tissues.
Primary cultures. Culture manipulation methods. In vitro and in vivo analysis of proliferative activity in healthy and neoplastic tissues. Cell kinetics: duration of the phases of cell cycle, potential and actual doubling time, growth fraction cell loss. Mathematical model for growth of healthy and neoplastic cell populations. Molecular basis of cell cycle regulation. Cell differentiation.
Growth of human solid tumours and parameters characterizing their aggressiveness. Biological prognostic parameters in human tumours.
Stem cells: sources, charcterization, repopulation after treatment with cytotoxic agents and use for therapeutic purpose.
Proliferative activity and turn over in tissue models. Effects of chemical, physical and biological agents on proliferation.